Alterations in intraocular cytokine levels following intravitreal ranibizumab



      Our previous work has shown that, after intravitreal bevacizumab (IVB) administration, decreases in the levels of vascular endothelial growth factor (VEGF)-A and placental growth factor (PlGF), along with increases in the levels of interleukin (IL)-8 and transforming growth factor (TGF)-β2, can be observed. It is not yet known if similar changes occur after intravitreal ranibizumab (IVR). The purpose of this study was to examine intraocular cytokine changes after IVR.


      Prospective clinical study.


      Subjects with proliferative diabetic retinopathy requiring pars plana vitrectomy (PPV) were recruited.


      Participants received IVR as pre-treatment before PPV. Aqueous humour levels of IL-8, VEGF-A, PlGF, and TGF-β2 were measured at time of pre-treatment and PPV. Results were analyzed using univariate statistical models.


      A total of 14 participants were recruited. After IVR administration, we observed a decrease in the levels of VEGF-A and PlGF, and an increase in the levels of IL-8 and TGF-β2. These results were statistically significant only for VEGF-A (p = 0.0001) and IL-8 (p = 0.0002).


      The changes in cytokine levels after IVR mirror the changes seen after IVB. Further studies are warranted in order to determine if there are any differences between IVB and IVR in this regard.


      Nos travaux antérieurs ont montré que, suite à l'administration de bevacizumab intravitréen (BIV), on constate une diminution des niveaux de FCEA-A et du facteur de croissance placentaire (PlGF), et une augmentation des niveaux d'IL-8 et de TGF-β2. Nous ne savons pas encore si des changements similaires se produisent suite à l'administration de ranibizumab intravitréen (RIV). Le but de l'étude était d'examiner les changements au niveau des cytokines intraoculaires après l'administration de RIV.


      Étude clinique prospective.


      Nous avons recruté des sujets atteints de rétinopathie diabétique proliférante (RDP) nécessitant une vitrectomie par la pars plana (VPP).


      Les participants ont reçu du RIV comme prétraitement avant de subir une VPP. Nous avons mesuré le niveau d'humeur aqueuse de l'IL-8, du FCEA-A, du PlGF et du TGF-β2 au moment du prétraitement et de la VPP. Nous avons analysé les résultats à l'aide de modèles statistiques univariés.


      Au total, 14 participants ont été recrutés. Après l'administration du RIV, nous avons observé une diminution des niveaux de FCEA-A et de PlGF, et une augmentation des taux d'IL-8 et de TGF-β2. Nous n'avons constaté des résultats statistiquement significatifs que pour le FCEA-A (p = 0,0001) et l'IL-8 (p = 0,0002).


      Les changements au niveau des cytokines après l'administration de RIV sont semblables aux changements observés après l'administration de BIV. D'autres études sont nécessaires afin de déterminer s'il y a des différences entre le BIV et le RIV à cet égard.
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