Abstract
Objective
Our previous work has shown that, after intravitreal bevacizumab (IVB) administration,
decreases in the levels of vascular endothelial growth factor (VEGF)-A and placental
growth factor (PlGF), along with increases in the levels of interleukin (IL)-8 and
transforming growth factor (TGF)-β2, can be observed. It is not yet known if similar changes occur after intravitreal
ranibizumab (IVR). The purpose of this study was to examine intraocular cytokine changes
after IVR.
Design
Prospective clinical study.
Participants
Subjects with proliferative diabetic retinopathy requiring pars plana vitrectomy (PPV)
were recruited.
Methods
Participants received IVR as pre-treatment before PPV. Aqueous humour levels of IL-8,
VEGF-A, PlGF, and TGF-β2 were measured at time of pre-treatment and PPV. Results were analyzed using univariate
statistical models.
Results
A total of 14 participants were recruited. After IVR administration, we observed a
decrease in the levels of VEGF-A and PlGF, and an increase in the levels of IL-8 and
TGF-β2. These results were statistically significant only for VEGF-A (p = 0.0001) and IL-8 (p = 0.0002).
Conclusions
The changes in cytokine levels after IVR mirror the changes seen after IVB. Further
studies are warranted in order to determine if there are any differences between IVB
and IVR in this regard.
Objectif
Nos travaux antérieurs ont montré que, suite à l'administration de bevacizumab intravitréen
(BIV), on constate une diminution des niveaux de FCEA-A et du facteur de croissance
placentaire (PlGF), et une augmentation des niveaux d'IL-8 et de TGF-β2. Nous ne savons
pas encore si des changements similaires se produisent suite à l'administration de
ranibizumab intravitréen (RIV). Le but de l'étude était d'examiner les changements
au niveau des cytokines intraoculaires après l'administration de RIV.
Nature
Étude clinique prospective.
Participants
Nous avons recruté des sujets atteints de rétinopathie diabétique proliférante (RDP)
nécessitant une vitrectomie par la pars plana (VPP).
Méthodes
Les participants ont reçu du RIV comme prétraitement avant de subir une VPP. Nous
avons mesuré le niveau d'humeur aqueuse de l'IL-8, du FCEA-A, du PlGF et du TGF-β2
au moment du prétraitement et de la VPP. Nous avons analysé les résultats à l'aide
de modèles statistiques univariés.
Résultats
Au total, 14 participants ont été recrutés. Après l'administration du RIV, nous avons
observé une diminution des niveaux de FCEA-A et de PlGF, et une augmentation des taux
d'IL-8 et de TGF-β2. Nous n'avons constaté des résultats statistiquement significatifs
que pour le FCEA-A (p = 0,0001) et l'IL-8 (p = 0,0002).
Conclusion
Les changements au niveau des cytokines après l'administration de RIV sont semblables
aux changements observés après l'administration de BIV. D'autres études sont nécessaires
afin de déterminer s'il y a des différences entre le BIV et le RIV à cet égard.
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References
- Alternative treatments to inhibit VEGF in age-related choroidal neovascularisation: 2-year findings of the IVAN randomised controlled trial.Lancet. 2013; 382: 1258-1267
- A prospective randomized trial of intravitreal bevacizumab versus ranibizumab for the management of diabetic macular edema.Am J Ophthalmol. 2013; 156: e502
- Lasting controversy on ranibizumab and bevacizumab.Theranostics. 2011; 1: 395-402
- Cytokine concentration in aqueous humour of eyes with exudative age-related macular degeneration.Acta Ophthalmol. 2012; 90: e381-e388
- Monocyte chemoattractant protein 1, intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 in exudative age-related macular degeneration.Arch Ophthalmol. 2010; 128: 1281-1286
- Soluble mediators of diabetic macular edema: the diagnostic role of aqueous VEGF and cytokine levels in diabetic macular edema.Curr Diab Rep. 2013; 13: 476-480
- Association of aqueous humor cytokines with the development of retinal ischemia and recurrent macular edema in retinal vein occlusion.Invest Ophthalmol Vis Sci. 2014; 55: 2290-2296
- Differences in aqueous concentrations of cytokines in macular edema secondary to branch and central retinal vein occlusion.PLoS One. 2013; 8: e68149
- Alterations in the intraocular cytokine milieu after intravitreal bevacizumab.Invest Ophthalmol Vis Sci. 2010; 51: 2388-2392
- Intravitreal bevacizumab increases intraocular interleukin-6 levels at 1 day after injection in patients with proliferative diabetic retinopathy.Cytokine. 2012; 60: 535-539
- Changes in aqueous cytokines after intravitreal triamcinolone versus bevacizumab for macular oedema in branch retinal vein occlusion.Acta Ophthalmol. 2014; 92: e217-e224
- Changes in the intraocular cytokine levels after intravitreal bevacizumab in uveitic macular edema.Ocul Immunol Inflamm. 2012; 20: 360-364
- Changes in aqueous concentrations of various cytokines after intravitreal triamcinolone versus bevacizumab for diabetic macular edema.Am J Ophthalmol. 2011; 152: 686-694
- Neovascular age-related macular degeneration: intraocular cytokines and growth factors and the influence of therapy with ranibizumab.Ophthalmology. 2009; 116: 2393-2399
- Synergism between vascular endothelial growth factor and placental growth factor contributes to angiogenesis and plasma extravasation in pathological conditions.Nat Med. 2001; 7: 575-583
- Binding and neutralization of vascular endothelial growth factor (VEGF) and related ligands by VEGF Trap, ranibizumab and bevacizumab.Angiogenesis. 2012; 15: 171-185
- Hypoxia inducible factor-1 independent pathways in tumor angiogenesis.Clin Cancer Res. 2007; 13: 5670-5674
- Induction of interleukin-8 preserves the angiogenic response in HIF-1alpha-deficient colon cancer cells.Nat Med. 2005; 11: 992-997
- Vitreous levels of placenta growth factor and vascular endothelial growth factor in patients with proliferative diabetic retinopathy.Diabetes Care. 2002; 25: 2352
- Concentration of cytokines in the aqueous humor of patients with naive, recurrent and regressed CNV associated with amd after bevacizumab treatment.Retina. 2009; 29: 523-529
- Cytokine concentration in aqueous humor of eyes with diabetic macular edema.Retina. 2012; 32: 2150-2157
Article info
Publication history
Accepted:
November 25,
2015
Received in revised form:
September 27,
2015
Received:
June 28,
2015
Identification
Copyright
© 2016 Canadian Ophthalmological Society. Published by Elsevier Inc. All rights reserved.
