Abstract
Objective
To investigate the agreement and correlation of manual central foveal thickness (CFT)
measurements using the Heidelberg Eye Explorer and ImageJ software.
Methods
Optical coherence tomography (OCT) images were identified. CFT was measured using
the Heidelberg Eye Explorer caliper tool. Two independent graders measured the CFT
of the same images using ImageJ software. A Pearson correlation coefficient, intraclass
correlation coefficient, Bland–Altman plots, and coefficient of repeatability were
used to assess the agreement and correlation between the Heidelberg Eye Explorer and
ImageJ measurements.
Results
One-hundred and twenty-two OCT images from 91 patients were analyzed. Mean CFT as
measured using the Heidelberg caliper tool was 264.8 ± 113 µm. Using ImageJ software,
graders 1 and 2 obtained mean CFT values of 267 ± 114.3 µm and 270 ± 114.8 µm, respectively.
Pearson correlation revealed a strong correlation between the Heidelberg and Image
J measurements (r = 0.999, p < 0.0001). Bland–Altman plots noted a less than 4 µm mean difference between Heidelberg
and ImageJ CFT values (2.67 ± 3.46 and 3.72 ± 2.78 µm for graders 1 and 2, respectively).
Conclusion
There was strong agreement between the Heidelberg Eye Explorer and ImageJ software
for manual CFT measurements. ImageJ may be a reliable tool for thickness measurements
when proprietary OCT software is unavailable.
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Article Info
Publication History
Published online: June 27, 2021
Accepted:
May 28,
2021
Received in revised form:
April 20,
2021
Received:
January 16,
2021
Publication stage
In Press Corrected ProofIdentification
Copyright
© 2021 Canadian Ophthalmological Society. Published by Elsevier Inc. All rights reserved.
