Abstract
Objective
To investigate the agreement and correlation of manual central foveal thickness (CFT)
measurements using the Heidelberg Eye Explorer and ImageJ software.
Methods
Optical coherence tomography (OCT) images were identified. CFT was measured using
the Heidelberg Eye Explorer caliper tool. Two independent graders measured the CFT
of the same images using ImageJ software. A Pearson correlation coefficient, intraclass
correlation coefficient, Bland–Altman plots, and coefficient of repeatability were
used to assess the agreement and correlation between the Heidelberg Eye Explorer and
ImageJ measurements.
Results
One-hundred and twenty-two OCT images from 91 patients were analyzed. Mean CFT as
measured using the Heidelberg caliper tool was 264.8 ± 113 µm. Using ImageJ software,
graders 1 and 2 obtained mean CFT values of 267 ± 114.3 µm and 270 ± 114.8 µm, respectively.
Pearson correlation revealed a strong correlation between the Heidelberg and Image
J measurements (r = 0.999, p < 0.0001). Bland–Altman plots noted a less than 4 µm mean difference between Heidelberg
and ImageJ CFT values (2.67 ± 3.46 and 3.72 ± 2.78 µm for graders 1 and 2, respectively).
Conclusion
There was strong agreement between the Heidelberg Eye Explorer and ImageJ software
for manual CFT measurements. ImageJ may be a reliable tool for thickness measurements
when proprietary OCT software is unavailable.
Objectif
Examiner la concordance et la corrélation des mesures manuelles de l’épaisseur centrale
de la fovéa réalisées avec les logiciels Heidelberg Eye Explorer et ImageJ.
Méthodes
On a mesuré, à partir d'images obtenues par tomographie par cohérence optique (OCT),
l’épaisseur centrale de la fovéa à l'aide de l'outil « calibre d’épaisseur » du logiciel
Heidelberg Eye Explorer. Deux évaluateurs indépendants ont ensuite mesuré l’épaisseur
centrale de la fovéa des mêmes images à l'aide du logiciel ImageJ. On a eu recours
à diverses méthodes (coefficient de corrélation de Pearson, coefficient de corrélation
intraclasse, méthode de Bland-Altman et coefficient de reproductibilité) pour mesurer
le degré de concordance et de corrélation entre les mesures obtenues avec les 2 logiciels
(Heidelberg Eye Explorer et ImageJ).
Résultats
Quelque 122 images d'OCT provenant de 91 patients ont été analysées. L’épaisseur centrale
de la fovéa moyenne selon le calibre d’épaisseur du logiciel Heidelberg Eye Explorer
se chiffrait à 264,8 ± 113 µm. Lorsqu'ils ont utilisé le logiciel ImageJ, les évaluateurs
1 et 2 ont obtenu une épaisseur centrale de la fovéa moyenne de 267 ± 114,3 µm et
de 270 ± 114,8 µm, respectivement. Selon le coefficient de corrélation de Pearson,
il est possible d’établir une corrélation fiable entre les mesures obtenues avec l'un
et l'autre logiciel (Heidelberg et ImageJ; r = 0,999; p < 0,0001). La méthode de Bland-Altman a mis au jour une différence moyenne de moins
de 4 µm entre les résultats du logiciel Heidelberg et ceux du logiciel ImageJ (2,67
± 3,46 µm et 3,72 ± 2,78 µm dans le cas des évaluateurs 1 et 2, respectivement).
Conclusion
On a constaté une forte concordance entre les logiciels Heidelberg Eye Explorer et
ImageJ pour ce qui est des mesures manuelles de l’épaisseur centrale de la fovéa.
Le logiciel ImageJ peut constituer un outil fiable de mesure de ce paramètre en l'absence
de logiciel d'OCT breveté.
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Canadian Journal of OphthalmologyAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Imaging of macular diseases with optical coherence tomography.Ophthalmology. 1995; 102: 217-229
- Trends in retina specialist imaging utilization from 2012 to 2016 in the United States Medicare fee-for-service population.Am J Ophthalmol. 2019; 208: 12-18
- Predictors of response to intravitreal anti–vascular endothelial growth factor treatment of age-related macular degeneration.Am J Ophthalmol. 2016; 163 (154–66.e8)
- Retinal thickness in diabetic retinopathy: a study using optical coherence tomography (OCT).Retina. 2002; 22: 759-767
- Foveal thickness can predict visual outcome in patients with persistent central serous chorioretinopathy.Ophthalmologica. 2009; 223: 28-31
- Long-term temporal changes of macular thickness and visual outcome after vitrectomy for idiopathic epiretinal membrane.Am J Ophthalmol. 2010; 150 (701–9.e1)
- NIH image to ImageJ: 25 years of image analysis.Nat Methods. 2012; 9: 671-675
- Measurement of foveal avascular zone dimensions and its reliability in healthy eyes using optical coherence tomography angiography.Am J Ophthalmol. 2016; 161 (50–5.e1)
- Optimization of ImageJ for automated image analysis to assess for photoreceptor cell death in retinal tissue sections.Invest Ophthalmol Vis Sci. 2014; 55: 4365
- A novel ImageJ macro for automated cell death quantitation in the retina.Invest Ophthalmol Vis Sci. 2015; 56: 6701-6708
- Image processing with ImageJ.Biophotonics Int. 2004; 11: 36-42
- Proposed lexicon for anatomic landmarks in normal posterior segment spectral-domain optical coherence tomography: the IN·OCT consensus.Ophthalmology. 2014; 121: 1572-1578
- A guideline of selecting and reporting intraclass correlation coefficients for reliability research.J Chiropr Med. 2016; 15: 155-163
- Reproducibility of macular thickness and volume using Zeiss optical coherence tomography in patients with diabetic macular edema.Ophthalmology. 2007; 114: 1520-1525
- Comparison of spectral/Fourier domain optical coherence tomography instruments for assessment of normal macular thickness.Retina. 2010; 30: 235
- Do different spectral domain OCT hardwares measure the same? Comparison of retinal thickness using third-party software.Graefes Arch Clin Exp Ophthalmol. 2015; 253: 1915-1921
- Refractory intraretinal or subretinal fluid in neovascular age-related macular degeneration treated with intravitreal ranizubimab: functional and structural outcome.Retina. 2015; 35: 1195-1201
- Insights into epiretinal membranes: presence of ectopic inner foveal layers and a new optical coherence tomography staging scheme.Am J Ophthalmol. 2017; 175: 99-113
- Errors in retinal thickness measurements obtained by optical coherence tomography.Ophthalmology. 2006; 113: 285-293
Article Info
Publication History
Published online: June 27, 2021
Accepted:
May 28,
2021
Received in revised form:
April 20,
2021
Received:
January 16,
2021
Identification
Copyright
© 2021 Canadian Ophthalmological Society. Published by Elsevier Inc. All rights reserved.
