Correspondence
Inadvertent corneal stromal staining by trypan blue following Descemet's membrane endothelial keratoplasty
Trypan blue is an azo dye solution that effectively stains basement membranes such as the anterior lens capsule and Descemet's membrane.1 In Descemet's membrane endothelial keratoplasty (DMEK), where the host Descemet's membrane and corneal endothelium are initially removed, trypan blue is used to stain the donor graft before injection to provide visibility during the unscrolling process in the anterior chamber. Trypan blue can also be injected after the descemetorrhexis and before graft injection to visualize any irregular tags or retained Descemet's membrane, both of which may increase the risk for graft detachment.Simple limbal epithelial transplantation to treat recurring kissing pterygium
Pterygium is a common ocular surface disorder characterized by chronic proliferative conjunctival fibrovascular tissue growth over the cornea.1 Numerous pterygium excision techniques have been tried with a wide range of recurrence rates, including bare sclera excision (30%–70%),2 amniotic membrane (6.4%–42.3%), and conjunctival autograft with or without glue (0%−16.7%).3,4 A recent Cochrane study found a 47% reduced risk of recurrence with conjunctival autograft at 6 months compared with amniotic membrane for pterygium excision.
